The present study aims at investigating difficulties of learning listening comprehension skill that face first-stage students who study English in the department of English/College of Education for Humanities/Anbar University and finding whether there is any significant difference in listening difficulties between males and females of the sample. The study is limited to the first-stage students who study English in the department of English/College of Education for Humanities/Anbar University in Ramadi city for the academic year (2017-2018). To achieve aims of the present study, two hypotheses are set. Firstly, it is hypothesized that first-stage students who study English in the department of English/College of Education for Humanities/Anbar University, face difficulties in learning listening comprehension skill. Secondly, it is hypothesized that there is a significant difference in the difficulties of learning listening comprehension skill between males and females of the sample according to the variable of gender at the level (0.05). The sample of this study includes 63 students (26 male and 37 female) who study English language in English department - College of Education for Humanities at Anbar University. The researcher used a questionnaire as the main instrument for collecting data. After subjecting the sample to the questionnaire, responses were analyzed by using means, standard deviations and percentages to achieve the first aim of the study. Moreover, t-test for independent samples was used to accomplish the second aim of this study. Results revealed that first stage English Department students in the College of Education for Humanities at Anbar University, face difficulties in learning listening comprehension skill. Also, it is found out that males and females of the sample face the same listening difficulties. Finally, on the basis of findings, the researcher offered suggestions, conclusions and recommendations.